Figure 1

Static H₂O₂ exposure results in heterogeneous and dose-dependent phosphorylation of BCR network proteins. (a) Schematic of the BCR network, with phosphatases and H₂O₂ influence. Kinases are indicated in red, phosphatases in green, sharp black arrows are activating effects, flat red arrows are inhibitory effects. (b) Density plots of PLCγ2 phosphorylation response to low (1 mM), medium (5 mM) or high (25 mM) static H₂O₂ doses after 0, 5, 10 or 25 min of exposure. Vertical lines indicate the ON/OFF threshold (see Methods for details). (c) pCD79a, pSYK and pPLCγ2 response over time (in percentage of cells responding) for different concentrations of H₂O₂ (average of 3 replicates per condition with standard deviation as error bars).