Figure 3

Dynamic H₂O₂-induced BCR signalling reveals that a combination of dose and rate of increase determine the response at intermediate doses. (a) Experimental set-up for creating dynamic gradient inputs. (b) Dynamic H₂O₂ input patterns. (c–e) pPLCγ2 signalling response upon gradient inputs over time (c), dose reached during gradients (percentage of gradient indicated) (d) and cumulative exposure (e). Blue = PBS step increase (negative control); green = H₂O₂ step increase (positive control); orange = 20 min linear gradient; dark purple = 60 min linear gradient; light purple = 60 min exponential gradient.