Table 3 Effect of in ovo feeding of methionine on the 17.5th day of incubation on blood biochemical parameters of TSL and HPC one-day chicks.

Plasma parameter		AST (U/L)	ALT (U/L)	AST: ALT	Uric acid (µmol/L)	FRAP (µM FeCl₂ equivalent)
Pooled effects
Genotype n = 32
	TSL	93.63	57.00	1.64^b	301.73^a	78.96^a
	HPC	98.08	59.50	2.02^a	267.67^b	70.27^b
Treatment n = 8
	Control	93.20^b	63.63	1.42^b	323.1^a	81.36^a
	Saline	109.13^a	49.90	2.81^a	283.99^ab	73.01^a
	DL-Met	88.11^b	63.59	1.43^b	284.22^ab	84.80^a
	L-Met	93.12^b	55.89	1.66^b	247.49^b	59.30^b
p-Values	Genotype	0.2750	0.6820	0.0340	0.0391	0.0171
	Treatment	0.0017	0.0563	0.0001	0.0110	0.0002
	Interaction	0.0007	0.0772	0.2331	0.0001	0.0021
RMSE		15.17	16.35	0.82	59.74	14.88
Treatment effect by genotype n = 8
TSL	Control	102.22	57.23	1.71	329.44^ab	90.12^a
	Saline	94.09	57.56	1.56	365.36^a	87.39^a
	DL-Met	87.54	63.47	1.42	276.59^b	78.21^a
	L-Met	91.19	49.73	1.87	235.54^c	60.12^b
	P-value	0.3200	0.3584	0.2361	0.0075	0.0069
	RMSE	15.97	15.024	0.4422	61.93	16.89
HPC	Control	84.18^b	70.02^a	1.12^b	316.78^a	72.60^b
	Saline	124.16^a	42.23^b	4.06^a	202.61^b	58.62^b
	DL-Met	88.67^b	63.71^ab	1.44^b	291.85^a	91.38^a
	L-Met	95.06^b	62.03^ab	1.45^b	259.43^ab	58.48^b
	P-value	0.0001	0.0267	0.0001	0.0035	0.0001
	RMSE	14.26	17.58	1.0761	57.81	12.34

Significant p-values are in [bold].
Means with similar superscripts are not significantly different within the column and effect (P > 0.05).
RMSE, root square of the mean square of error; TSL, TETRA SL genotype; HPC, Hungarian partridge colored hen genotype; AST, aspartate aminotransferase; ALT, alanine aminotransferase—AST; ALT, aspartate aminotransferase alanine aminotransferase ratio; FRAP, ferric reducing ability of the plasma.

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