Figure 3
From: Kinetic characterization of human mRNA guanine-N7 methyltransferase
Amenability of the optimized assay for screening. (A) Sinefungin, (B) Suramin, and (C) SAH inhibited RNMT with IC50 values of 3 ± 0.6 nM (Hill Slope: 1.2), 5 ± 0.4 nM (Hill Slope: 0.8) and 389 ± 110 nM (Hill Slope: 0.9), respectively. Plotted values are the mean ± standard deviation of three independent experiments. Data were analyzed using GraphPad Prism software 9. (D) The Z-factor was determined in the absence (black rectangles) and presence (blue circles) of 50 μM Sinefungin using the optimized screening conditions.
