Table 1 Kinetic parameters of RNMT. All experiments were performed in triplicate, and data are shown as the mean ± standard deviation.

From: Kinetic characterization of human mRNA guanine-N7 methyltransferase

RNMT

Substrate

Kmapp (nM)

kcatapp (h-1)

kcat/Km (h-1 nM-1)

RNMT (1–476)

3H-SAM

190 ± 38

93 ± 8

0.48

RNA

158 ± 8

91 ± 1.2

0.58

RNMT (123–476)

3H-SAM

196 ± 10

41 ± 2

0.21

RNA

75 ± 11

39 ± 2

0.52

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