Figure 2 | Scientific Reports

Figure 2

From: StayGold photostability under different illumination modes

Figure 2

Effects of chemical fixation on the fluorescence of nuclear-targeted FPs. Time-lapse imaging of HeLa cells expressing H2B-StayGold, H2B-EGFP, H2B-mClover3 and H2B-mNeonGreen in HBSS. left, Representative low-magnification fluorescence images before fixation. Scale bars, 50 µm. right, Fluorescence intensities (FIs) of several cells are individually plotted against time. Within several hours after washing with HBSS, FIs of recovered fluorescence reached their plateaus. See Supplementary Fig. S1 for the mNeonGreen fluorescence.

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