Figure 5
From: StayGold photostability under different illumination modes
Photostability of StayGold, EGFP, mClover3 and mNeonGreen fused to H2B in living HeLa cells in HBSS with multi-beam LSCM. Comparison of the photostability of the four green-emitting FPs in living cells in each experiment using multi-beam LSCM. The scan-averaged irradiance value and disk rotation speed are shown at the upper right. Photobleaching curves are calculated based on the irradiance and FP molecular brightness (Table 1), plotted as intensity versus normalized total exposure time with an initial emission rate of 1000 photons/s/molecule. Data points are shown as means (n = 5 cells). The transit time is 3.1–8.7 µs (4000 rpm) or 8.3–23.2 µs (1500 rpm).
