Table 1 Photostability of StayGold and reference green-emitting FPs.

From: StayGold photostability under different illumination modes

Illumination

Köhler

Critical

Illumination mode

WF

Single-beam LSCM

Spinning disk LSCM

Transit time (μs)

Rotation speed (rpm)

4.6

0.92

0.031

3.7

4000

1500

Radiant flux (mW)

5.2

4.3/4.5

0.18

0.12

0.079

0.15

3.1

3.1

Area (cm2)

0.002375

0.002375

0.001013

0.001013

0.001013

0.000253

0.002139

0.002139

Irradiance (W/cm2)

2.2

1.8/1.9

0.178

0.118

0.078

0.593

1.45

1.45

t1/2 (s)

 StayGold

6847

12,098

204

145

138

179

1,883

1,947

 EGFP

198

288

64

70

64

48

327

338

 mClover3

44

47

49

45

71

33

72

72

 mNeonGreen

138

167

47

31

46

35

182

209

 Related Fig

Figure 3

Suppl. Fig. S2

Figure 4

Figure 5

  1. The time for photobleaching from an initial emission rate of 1000 photons/s/molecule down to 500 (t1/2) in each experiment is shown. Scan-averaged irradiance was measured in LSCM (critical illumination). All values were measured in this study.
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