Figure 4 | Scientific Reports

Figure 4

From: Study of the mechanism by gentiopicroside protects against skin fibroblast glycation damage via the RAGE pathway

Figure 4

GPS regulates the synthesis of ECM proteins and regulates the expression of vimentin. (A) qRT-PCR assay to detect the expression of ECM protein mRNAs. (B) WB assay to detect the expression of ECM proteins. (C) ELISA to detect the expression of ECM proteins. (D) qRT-PCR assay to detect the expression of MMP-2 and MMP-9 mRNAs. (E) Immunofluorescence assay to detect vimentin protein expression. All the target investigated genes and housekeeping genes were counted on the same WB membrane for statistical examination.

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