Figure 2
(a, c) Effect of UV-C radiation, Ps80 and US on the viability of bacteria (S. aureus) during 4 h of circulation through the decontamination system. The axes represent UV-C fluence delivered in J cm−2 and time in minutes, with results significantly differing from the control. Negative control samples (untreated) are represented as open black circles, UV-C and US combination samples as solid black triangles, UV-C and Ps80 combination samples as solid black squares, and the combination of all techniques (UV-C + Ps80 + US) as solid black diamonds. The experiment was performed in triplicate on three different occasions (n = 9), and mean values with standard deviation are presented. (b, d) Bacteria recovered from infection with S. aureus in the liquid. Initial S. aureus inoculum, T0, represents HTK with S. aureus bacteria after 1 h of infusion, and the macerate of kidney samples after decontamination. The experiment performed in triplicate on three different occasions (n = 9).
