Figure 3 | Scientific Reports

Figure 3

From: Three-dimensional identification of microvascular pathology and neurovascular inflammation in severe white matter hyperintensity: a case report

Figure 3

Cubes preparation for immunolabeling and clearing protocol. (a) Axial tissue slab corresponding to the region of interest used for immunolabeling and clearing. The white grid illustrates how the slab was initially cut into several rows. Dashed rectangle displays tissue blocks in (b). White lines in (b) show the section areas to produce the cubes shown in (c). The black arrows in (c) point to the cubes shown in (d) after immunolabeling for both glucose transporter 1 (GLUT1) and ionized calcium-binding adapter molecule 1 (IBA1), clearing and imaging. Scale bars: 0.5 cm (a–c), 500 µm (d).

Back to article page