Figure 1

(a) The structure of the micropore module. The micropore module is 25 mm2 in size and 0.5 mm thick. The bacterial suspension is introduced from the cis channel, and PBS is introduced from the trans channel. (b) An optical image of the pore of the module. The optical microscopic examination of the micropore module suggests that the 3 μm diameter micropore is in the centre of the silicon substrate. (c) A schema of the micropore. When the bacteria pass through the micropore, the ionic current decreases because of obstruction in flowing ions. The processing software obtains the change in ionic current as a waveform. (d) Scanning electron microscope (SEM) observation of S. epidermidis ×14,000. (e) SEM observation of S. epidermidis ×60,000. (f) SEM observation of S. aureus ×14,000. (g) SEM observation of S. aureus ×60,000. (h) A scatter diagram of the OD600 of the bacterial suspensions and their average pulse counts. No correlations were observed between the OD600 and pulse counts of the bacterial suspensions.