Figure 9

Scene cell instance segmentation and tracking. The scene instance-level segmentation module can use the DL module (Fig. 5b) or the IDL module (Fig. 5c) for a scene cell instance segmentation. This module quantifies cell count, area and coordinates for each frame. Cell speed and total movement quantification loads the scene cell features (frame id, centroid, area). Cell centroids are corrected using scene shift correction module (Fig. 4b). For cell speed and total movement quantification, any tracking algorithm (i.e., Bayesian Tracker) can be applied on the corrected cell features. Results of all complementary modules are saved in an open-source format (i.e., CSV).