Figure 2

Growth and biological characteristics of hUC-MSCs. (A) hUC-MSC growth and morphology were observed under an inverted microscope (× 4). The left panel shows a few adherent cells on day 3 of culture. The middle graph shows that many cells migrated after 5–9 days of culture. The figure on the right shows that the cell morphology at passage 4 was uniform and the cells had a short spindle shape with vortex growth. (B) Flow cytometry was used to analyse cell surface antigens of human umbilical cord mesenchymal stem cells. In the figure, from left to right, are the column diagrams of the SSC-FSC double-scattered light parameters, FITC-CD34, APC-CD45, FITC-CD90, PE-CD73, and APC-CD105.