Figure 2
From: High-throughput homogenous assay for the direct detection of Listeria monocytogenes DNA
Sequence alignment and primer location. Nucleic acid alignment of the 16S rDNA region for L. monocytogenes and the two most closely related species (L. innocua and L. ivanovii) with nucleotide difference noted in red (top) along with a schematic of the locations of the different oligos used during assay development (bottom). Stars denote nucleotide differences with oligos demarcated by blue lines corresponding to donor oligos and those demarcated by red lines corresponding to acceptor oligos.
