Figure 3
From: High-throughput homogenous assay for the direct detection of Listeria monocytogenes DNA
Specificity of oligo-Alpha using oligos with 1 vs 2 nucleotide differences. The ability to specifically detect L. monocytogenes was assessed using 90 ng of Listeria DNA, a donor oligo containing either 1 SNP (right) or 2 SNPs (left), and the L. mono_16S-Rev7 acceptor oligo. Averages based on four independent trials are shown with signals that are statistically higher (p ≤ 0.05) than the no DNA control denoted by an *. Error bars denote the standard deviation of the mean.
