Figure 1
From: Overcoming radioresistance of breast cancer cells with MAP4K4 inhibitors
Overexpression of MAP4K4 in radioresistant breast cancer cell lines derived from SK-BR-3 and MCF-7 cells. (A) Clonogenic survival assays after irradiating cells with 1, 2, 3, 4, or 5 Gy. Radioresistant SK-BR-3 (SR) cells and radioresistant MCF-7 (MR) cells were resistant to radiation as compared with parental cells (SK-BR-3 and MCF-7). (B) Western blot revealed MAP4K4 expression was markedly elevated in SR and MR cells. MAP4K4 expression levels are expressed as fold changes versus parental cells. (C) Immunofluorescence analysis of MAP4K4 in parental (SK-BR-3 and MCF-7) and radioresistant (SR and MR) cells. (D) Western blot analysis of p-ERK 1/2, ERK 1/2, p-JNK 1/2, JNK 1/2, p-p38, and p38 in SR and MR cells versus parental cells. p-ERK 1/2, ERK 1/2, p-JNK 1/2, JNK 1/2, p-p38, and p38 protein levels are expressed as fold changes versus parental cells. Graphs were plotted using the means ± SDs of three independent experiments. * and ** indicate P values of < 0.05 and P < 0.01 versus parental cells, respectively.
