Figure 5
From: Overcoming radioresistance of breast cancer cells with MAP4K4 inhibitors
MAP4K4 functioned as an upstream effector of ACSL4. (A) Immunofluorescence analysis of MAP4K4 and ACSL4 in SR and MR cells indicated that MAP4K4 co-localized with ACSL4 in radioresistant cells. (B) Expressions of ACSL4 in SR cells treated with PF06260933 or GNE-495. (C) Expressions of ACSL4 and MAP4K4 in SR cells treated with triacsin C (ACSL4 inhibitor). (D) Effect of triacsin C on MAP4K4 kinase activity. The protein expressions of MAP4K4 and ACSL4 following siRNA knockdown. MAP4K4 (E) and ACSL4 (F) protein levels are expressed as fold changes versus untreated cells (0 µM). Results are presented as means ± SDs, and * and ** indicate P values of < 0.05 and < 0.01, respectively.
