Figure 2

miR-181a overexpression protects mouse hearts from MI-induced LV remodeling. (a) Representative end-systolic short-axis cine MR images of WT and miR-181a Tg mice at 4 weeks after MI, respectively. (b) MRI analysis LV dimensions and cardiac function in WT and miR-181a Tg mice at 4 w after sham or MI. LVEF, left ventricular ejection fraction; LVFS, left ventricular fractional shortening; LVIDd, diastolic left ventricular internal diameters; LVIDs, systolic left ventricular internal diameters; SV, systolic volume. *P < 0.05 compared with WT subjected to MI; n = 6 mice per group. (c) Representative photographs and quantitative data of Masson’s trichrome staining of heart sections for collagen 4 weeks after MI with WT and miR-181a Tg mice. *P < 0.05 compared with WT subjected to MI; Scale Bar, 1.5 mm. n = 6 mice per group. (d) Heart/body weight ratio of WT and miR-181a Tg mice subjected to 4 weeks MI or sham. *P < 0.05 compared with WT subjected to MI; n = 6 mice per group. (e) Kaplan–Meier survival curves of WT and miR-181a Tg mice subjected to 4 weeks MI. *P < 0.05 compared with WT subjected to MI; n = 20 mice per group. (f) qRT-PCR showing mRNA levels of col1a1, col3a1, α-SMA in hearts of WT or miR-181a Tg mice subjected to 4 weeks MI or sham. *P < 0.05 compared with WT subjected to MI; n = 6 mice per group.