Figure 5

miR-181a regulates apoptosis and mitochondrial fission through PDCD4 and BID. (a) Representative TEM images of mitochondria from miR-181a Tg mice or WT mice subjected to MI, Scale bar, 0.5 μm; arrows indicate fission mitochondria. n = 6 mice per group. (b) Representative mitochondrial morphology images in cardiomyocytes infected with adenoviral miR-181a or miR-181a antagomir (up). Original magnification, × 400. Quantitative analysis of mitochondrial fission is shown at down. *P < 0.05 compared with anoxia alone. (c) Representative images of subcellular localization of BID in NRVCs transfected with adenoviral miR-181a or miR-181a antagomir and then exposed to anoxia. Original magnification, × 400. (d) Expression of BID protein was detected using western blot assay. *P < 0.05 compared with adenoviral miR-181a along with PDCD4-3’UTR-mut. (e) Mitochondrial fission in NRVCs upon to anoxia co-transfected with adenoviral miR-181a in combination with PDCD4-3′UTR or PDCD4-3′UTR-mut. *P < 0.05 compared with adenoviral miR-181a along with PDCD4-3′UTR-mut. (f) Apoptotic cells in NRVCs upon to anoxia co-transfected with adenoviral miR-181a in combination with PDCD4-3′UTR or PDCD4-3′ UTR-mut. *P < 0.05 compared with adenoviral miR-181a along with PDCD4-3’UTR-mut.