Figure 4

Hybrid cell subpopulations. (A, B) K-means cluster analysis of normalized fluorescence intensities from identified hybrid cells in colorectal cancer (CRC) and pancreatic cancer (PDAC) tumor sections and peripheral blood depicted as a heat map. Each column represents a single hybrid cell (CD45⁺ and ECAD⁺, EpCAM⁺, or panCK⁺) and each row represents the normalized fluorescent intensity of an antigen in the Ab-oligo panel. Cells were grouped by subpopulation. (C) Pairwise correlation analysis between hybrid cell clusters in the tumor section and those in the blood in CRC and PDAC patient matched specimens. αSMA alpha smooth muscle actin, CK cytokeratin; CRC colorectal cancer, ECAD E-cadherin, EGFR epidermal growth factor receptor; EpCAM epithelial cell adhesion molecule, pAKT phosphorylated protein kinase, strain AK, Thymoma (phosphorylated protein kinase B); panCK pan-cytokeratin PDAC pancreatic ductal adenocarcinoma, VIM vimentin, VWF von Willebrand factor.