Figure 3 | Scientific Reports

Figure 3

From: HOIL-1L deficiency induces cell cycle alteration which causes immaturity of skeletal muscle and cardiomyocytes

Figure 3

RNA-seq analysis of Hoil-1l KO C2C12 shows mitotic accumulation in Hoil-1l KO C2C12 myotubes. (A) GSEA identified a significant enrichment of gene set associated with G2M checkpoint and mitotic spindle in Hoil-1l KO C2C12 myotubes. (B) Immunostaining of histone H3 phosphorylation (pHH3) of C2C12 myoblasts showed more PHH3 positive Hoil-1l KO C2C12 myoblasts. From top to bottom, they are merged, pHH3 signal only and magnified images, respectively. Bar = 50 μm. (N = 3 independent experiments, All data are presented as mean ± SEM. P values from Welch’s t-test. WT vs Exon5 KO and Exon7 KO. **P < 0.01, ***P < 0.001). (C) Heatmaps of the G2M check point associated genes enriched in Hoil-1l KO C2C12 myotubes compared with WT C2C12 myotubes. Especially, CDK1, AURKB, CDC20 and CCNB2 (noted with red color; cyclin family), which promote entry into mitosis and maintain of the mitosis state.

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