Figure 1

Optimizing the amino acid content of 100N for Drosophila pre-adulthood. (a), Eclosion kinetics, median egg-to-adult duration in days after-egg-lay (dAEL), mean percentage egg-to-adult survivorship, and male weight upon eclosure (mg) on SY and 100N diets. (b–d), Egg-to-adult duration (dAEL) and egg-to-adult survivorship on 100N supplemented with (b) 0.1%; 0.5%; or 1% of yeast extract (YE), (c) 50% Ser; 50% Gly; 100% Glu; 50% supplementation of all 20 aa (150N); and (d) 50% Ser, Val and Gly, 50% Gly and Ser, 110% Glu, and 8% supplementation of all 20 aa (108N). (a–d), five biological replicates per group of 20 individuals each. For adult male weight upon eclosure at least 30 individuals were weighted per group. (a), Student's unpaired t-test, ****p < 0.0001, *p < 0.05. b-d, One-way ANOVA followed by Tukey’s HSD test; different letters (A, B and C) represent statistically significant differences (P < 0.05).