Figure 2
Metabolic profile comparison of 3rd instar larvae reared on SY or 108N. (a), Principal Component Analysis (PCA) of metabolic profiles of third instar larvae reared on SY or 108N. For metabolome analysis a total of five biological replicates were performed per group, each replicate containing 10 five-day-old larvae. (b,c) Log2(fold change) of larval metabolites involved in (b) long-chain FA transport in mitochondria, and (c) energy metabolism significantly affected in 108N in comparison to SY media. Student's unpaired t-test, ****p < 0.0001. (d), Top 50 metabolites, based on fold change, significantly affected by dietary treatment (Student's unpaired t-test, P < 0.05). The cell color represents the z-score, i.e., the standardized scores on the same scale, calculated dividing a score's deviation by the standard deviation in the row. The features are color-coded by row with red indicating high intensity and blue indicating low intensity. (e,f), Enrichment analysis showing the top 20 pathways (based on small molecule pathway database—SMPDB) (e) increased or (f) decreased in larvae reared on 108N in comparison to larvae reared on SY.
