Figure 3
Optimizing the 108N diet for D. melanogaster pre-adulthood. (a), Egg-to-adult duration (dAEL) and egg-to-adult survivorship on 108N containing 0.45%; 0.6%; or 0.75% acetic acid (AcOH, v/v). (b), Lipid storage in the late-larval fat body on 100N, 108N (0.6% AcOH), or SY. Nucleic acids (blue) and lipid droplets (red), 400 × magnification. (c), Percentage of fat body area occupied by lipid droplets. (d), Adult male weight upon eclosion (mg) raised on SY, 100N, or 108N (0.6% AcOH). (e), Lifespan curves of adult flies fed SY food after being raised on either 108N (0.6% AcOH) or SY during their pre-adult period. (f), Eclosion kinetics, egg-to-adult duration (dAEL) and egg-to-adult survivorship on SY, 100N, and 108N (0.6% AcOH) diets. (a) and (f), Five biological replicates per group of 20 individuals each. (c), Five larvae per group (each point represents an average of 5 individual measurements per larva). (d), For adult male weight upon eclosure at least 30 individuals were weighed per group. (e), 5 to 10 replicates per group of 10 female adults each. (a), (c), (d), and (f), One-way ANOVA followed by Tukey’s HSD test; different letters (A, B and C) represent statistically significant differences (p < 0.05). i, Cox Proportional-Hazards modelling (p = 0.472). Data shown in Fig. 1a are repeated in Fig. 3d and f, to compare against 108N (0.6% AcOH).
