Figure 1

scRNA-seq analysis identifies NK cell marker genes. (A) Quality control of scRNA-seq data from eight GC samples. (B) The variance plot showed 1500 genes in all cells, red dots represent the top 1500 highly variable genes. (C) PCA was utilized for dimensionality reduction. (D) 15 PCs were identified based on P-value < 0.05. (E) t-SNE plot colored by various cell clusters. (F) Heatmap showing the top marker genes in each cell cluster. (H) The cell subpopulations identified by marker genes.