Figure 2 | Scientific Reports

Figure 2

From: Homocysteine metabolites inhibit autophagy by upregulating miR-21-5p, miR-155-5p, miR-216-5p, and miR-320c-3p in human vascular endothelial cells

Figure 2

Influence of N-Hcy-protein, Hcy-thiolactone, and Hcy on the expression of autophagy-related mRNAs in HUVEC. Bar graphs show levels of BECN1 mRNA (A), ATG5 mRNA (B), ATG7 mRNA (C), LC3 mRNA (D), and p62 mRNA (E) in HUVEC treated with N-Hcy-protein (N-Hcy), Hcy-thiolactone (HTL), or Hcy in methionine-free M199/dialyzed FBS medium for 24 h. Showed mRNAs were quantified by RT-qPCR with GAPDH mRNA as a reference. Each assay was repeated three times (technical repeats) in three independent experiments (biological repeats). Mean ± SD values of three biological repeats for each treatment group are shown. *P < 0.05, **P < 0.01, ***P < 0.001 from one-way ANOVA with Tukey’s multiple comparisons test.

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