Figure 5 | Scientific Reports

Figure 5

From: Homocysteine metabolites inhibit autophagy by upregulating miR-21-5p, miR-155-5p, miR-216-5p, and miR-320c-3p in human vascular endothelial cells

Figure 5

miR inhibitors abrogate stimulatory effects of N-Hcy-protein, Hcy-thiolactone, and Hcy on autophagy-related mRNA expression. Bar graphs show levels of BECN1 mRNA (A,B), LC3 mRNA (C–E), p62 mRNA (F), ATG5 mRNA (G,H), and ATG7 mRNA (I) in HUVEC treated with N-Hcy-protein (N-Hcy), Hcy-thiolactone (HTL), or Hcy in HUVEC transfected with mirVana™ miRNA Mimic, Negative Control #1 (Control) or corresponding miR inhibitors for 4 h. The cells transfected with a miR inhibitor were then untreated (Control+) or treated with N-Hcy-protein (N-Hcy), Hcy-thiolactone (HTL), or Hcy in methionine-free M199/dialyzed FBS medium for 24 h. Levels of mRNAs were quantified by RT-qPCR with GAPDH mRNA as a reference. Each assay was repeated three times (technical repeats) in three independent experiments (biological repeats). Mean ± SD values of three biological repeats for each treatment group are shown. *P < 0.05, **P < 0.01, ***P < 0.001 from Student’s two-tailed t test.

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