Figure 6 | Scientific Reports

Figure 6

From: Homocysteine metabolites inhibit autophagy by upregulating miR-21-5p, miR-155-5p, miR-216-5p, and miR-320c-3p in human vascular endothelial cells

Figure 6

Influence of N-Hcy-protein, Hcy-thiolactone, and Hcy on the expression of autophagy-related proteins in HUVEC in the presence of a corresponding miR inhibitor. Bar graphs show levels of BECN1 protein (A,B), LC3-II protein (C–E), LC3-II/LC3-I ratio (F–H), LC3-I (I), p62 protein (J), ATG5 protein (K,L), and ATG7 protein (M) in HUVEC transfected with mirVana™ miRNA Mimic, Negative Control #1 (Control) or a corresponding miR inhibitor for 4 h. The cells transfected with a miR inhibitor were then untreated (Control+) or treated with N-Hcy, HTL, or Hcy in methionine-free M199/dialyzed FBS medium for 24 h. Indicated proteins were quantified by Western blotting with GAPDH protein as a reference; representative images are shown in panels (N–Q). Each assay was repeated three times (technical repeats) in three independent experiments (biological repeats). Mean ± SD values of three biological repeats for each treatment group are shown. *P < 0.05, **P < 0.01, ***P < 0.001 from a Student’s two-tailed t test.

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