Table 1 Ingredients and chemical composition of the experimental diets.

From: Dietary tryptophan intervention counteracts stress-induced transcriptional changes in a teleost fish HPI axis during inflammation

Ingredients (%)

CTRL

TRP

CPSP 90a

5.00

5.00

Fish gelatinb

2.00

2.00

Soy protein concentratec

25.00

25.00

Pea protein concentrated

6.00

6.00

Wheat glutene

10.00

10.00

Corn gluten mealf

15.00

15.00

Wheat mealg

15.80

15.50

Vitamins and minerals premixh

1.00

1.00

Antioxidanti

0.20

0.20

Sodium propionatej

0.10

0.10

MCPk

3.00

3.00

l-Lysine HCl 99%l

0.60

0.60

l-Tryptophanm

0.00

0.30

dl-Methioninen

0.20

0.20

Soy lecithino

1.00

1.00

Fish oilp

15.10

15.10

Total

100.00

100.00

Proximate analysis (% dry weight)

 Crude protein

45.70

46.00

 Crude fat

18.00

18.00

 Fiber

1.70

1.70

 Starch

13.40

13.20

 Ash

6.80

6.80

 Energy (MJ kg−1)

21.90

21.90

  1. aCPSP 90: 82.6% crude protein (CP), 9.6% crude fat (CF), Sopropêche, France.
  2. bFish gelatin: 88% CP, 0.1% CF, LAPI Gelatine SPA, Italy.
  3. cSoycomil P: 63% CP, 0.8% CF, ADM, The Netherlands.
  4. dNUTRALYS F85F: 78% CP, 1% CF, ROQUETTE Frères.
  5. eVITAL: 83.7% CP, 1.6% CF, ROQUETTE Frères, France.
  6. fCorn gluten meal: 61% CP, 6% CF, COPAM, Portugal.
  7. gWheat meal: 10.2% CP; 1.2% CF, Casa Lanchinha, Portugal.
  8. hPREMIX Lda, Portugal: Vitamins (IU or mg kg−1 diet): dl-alpha tocopherol acetate, 100 mg; sodium menadione bisulphate, 25 mg; retinyl acetate, 20,000 IU; dl-cholecalciferol, 2000 IU; thiamin, 30 mg; riboflavin, 30 mg; pyridoxine, 20 mg; cyanocobalamin, 0.1 mg; nicotinic acid, 200 mg; folic acid, 15 mg; ascorbic acid, 500 mg; inositol, 500 mg; biotin, 3 mg; calcium panthotenate, 100 mg; choline chloride, 1000 mg, betaine, 500 mg. Minerals (g or mg kg-1 diet): copper sulphate, 9 mg; ferric sulphate, 6 mg; potassium iodide, 0.5 mg; manganese oxide, 9.6 mg; sodium selenite, 0.01 mg; zinc sulphate, 7.5 mg; sodium chloride, 400 mg; excipient wheat middlings.
  9. iParamega PX, Kemin Europe NV, Belgium.
  10. jPREMIX Lda., Portugal.
  11. kMonocalcium phosphate: 22% phosphorus, 16% calcium, Fosfitalia, Italy.
  12. ll-Lysine HCl 99%, Ajinomoto Eurolysine SAS, France.
  13. ml-Tryptophan 98%, Ajinomoto Eurolysine SAS, France.
  14. ndl-Methionine for Aquaculture: 99% Methionine, Evonik Nutrition & Care GmbH, German.
  15. oLecico P700IPM, LECICO GmbH, Germany.
  16. pSAVINOR UTS, Portugal.