Table 3 Oligonucleotide sequences used to evaluate relative mRNA abundance of genes in the hypothalamus, pituitary gland and head-kidney by RT-qPCR, including forward (F) and reverse (R) primers, GenBank ID (NCBI), efficiencies (E) of qPCR reactions and annealing temperature (Ta).

From: Dietary tryptophan intervention counteracts stress-induced transcriptional changes in a teleost fish HPI axis during inflammation

Gene	Acronym	GenBank ID	E	Ta (ºC)	Primer sequence (5′–3′)
Hypothalamus
Elongation factor 1-α	ef1α	AJ866727.1	2.31	60	F: AACTTCAACGCCCAGGTCAT R: CTTCTTGCCAGAACGACGGT
40s ribosomal protein	40s	HE978789.1	2.23	60	F: TGATTGTGACAGACCCTCGTG R: CACAGAGCAATGGTGGGGAT
Interleukin 1 β	il1β	AJ269472.1	2.04	60	F: AGCGACATGGTGCGATTTCT R: CTCCTCTGCTGTGCTGATGT
Interleukin 6	il6	AM490062.1	2.04	55	F: AGGCACAGAGAACACGTCAAA R: AAAAGGGTCAGGGCTGTCG
Interleukin 10	il10	AM268529.1	2.06	55	F: ACCCCGTTCGCTTGCCA R: CATCTGGTGACATCACTC
Glucocorticoid receptor 1	gr1	AY619996.1	2.13	60	F: AAATCTGCCTGGTGTGTTCC R: TGCCCTTTCACTGCTCTCTT
Glucocorticoid receptor 2	gr2	AY549305.1	2.18	60	F: CTTCTACAGCACCAGCACCA R: TCTCCTGTTTGACCACACCA
Corticotropin releasing hormone-binding protein	crhbp	MG832822.1	2.09	60	F: TGTCATCTCCCAGTCACCAG R: GCCATTTCCTCCAAGCAAC
Corticotropin releasing hormone	crh	JF274994.1	1.97	60	F: AACCCAAAACTCCCAGCAG R: TGTTCCCAACTTTCCCTTGT
5-hydroxytryptamine (serotonin) receptor 1A β-like	htr1aβ	DLAgn_00119560^a	2.33	60	F: GGAGCGTAAAACGGTGAAAA R: TGGGGTTGAGGAGAGAGTTG
Macrophage colony-stimulating factor 1 receptor 1	mcsf1r1	DLAgn_00109630^a	2.26	61	F: ATGTCCCAACCAGACTTTGC R: GGCTCATCACACACTTCACC
Tumor necrosis factor-alpha	tnfα	DQ070246.1	2.03	55	F: AGCCACAGGATCTGGAGCTA R: GTCCGCTTCTGTAGCTGTCC
Transforming growth factor-beta	tgfβ	AM421619.1	2.04	55	F: ACCTACATCTGGAACGCTGA R: TGTTGCCTGCCCACATAGTAG
Tryptophan 5-hydroxylase-like	tph1α	DLAgn_00154580^a	1.98	60	F: CGCATAGACTTCACAACAGAGG R: CAGCAGAGGGAGGTTCTTCA
μ opioid receptor	muor	DLAgn_00015310^a	2.04	60	F: GTCACCAGCACCCTACCATT R: CGAGGAGAGAATCCAGTTGC
κ-type opioid receptor-like 2	kor2	DLAgn_00007470^a	1.96	60	F: TCTGGTGCTTGTGGTAGTCG R: TGGCAGTCTCTGTGTCCTTG
δ-opioid receptor	dor2	DLAgn_00062690^a	2.12	60	F: CGCTTCTCGGTCTCCATAACT R: GGTCTCATTACTACTTGAAG
Opioid growth factor receptor 2	ogfr2	DLAgn_00128530^a	2.23	60	F: GTTGGGAATGGAGATGGAAA R: GCTTCAGATTTTGGCTCAGG
Pituitary gland
Elongation factor 1-α	ef1α	AJ866727.1	2.35	60	F: AACTTCAACGCCCAGGTCAT R: CTTCTTGCCAGAACGACGGT
40s ribosomal protein	40s	HE978789.1	2.48	60	F: TGATTGTGACAGACCCTCGTG R: CACAGAGCAATGGTGGGGAT
Interleukin 1 β	il1β	AJ269472.1	2.17	60	F: AGCGACATGGTGCGATTTCT R: CTCCTCTGCTGTGCTGATGT
Glucocorticoid receptor 1	gr1	AY619996.1	2.24	60	F: AAATCTGCCTGGTGTGTTCC R: TGCCCTTTCACTGCTCTCTT
5-hydroxytryptamine (serotonin) receptor 2a	htr2a	DLAgn_00222310^a	2.19	60	F: CCTCTGACCTCTGTCCCATC R: ACTGAAATCGTCCACACTGC
Tryptophan 5-hydroxylase-like	tph1α	DLAgn_00154580^a	2.01	60	F: CGCATAGACTTCACAACAGAGG R: CAGCAGAGGGAGGTTCTTCA
Pro-opiomelanocortin a-like	pomca	AY691808.1	2.28	60	F: TCTTCCTCCTCCTCTCCACA R: CGCCTTCTCATCTCTTCAGG
Pro-opiomelanocortin b-like	pomcb	DLAgn_00069720^a	1.97	60	F: GGTTGTTAGTGGTGGTGATGG R: GTCCTTGTTGCTCAGGTCGT
Head-kidney
Elongation factor 1-α	ef1α	AJ866727.1	2.35	57	F: AACTTCAACGCCCAGGTCAT R: CTTCTTGCCAGAACGACGGT
40s ribosomal protein	40s	HE978789.1	2.48	60	F: TGATTGTGACAGACCCTCGTG R: CACAGAGCAATGGTGGGGAT
Glucocorticoid receptor 1	gr1	AY619996.1	2.24	60	F: AAATCTGCCTGGTGTGTTCC R: TGCCCTTTCACTGCTCTCTT
Melanocortin 2 receptor	mc2r	DLAgn_00065140^a	2.01	60	F: GAGGGCAAGGGGAGCATTTA R: GACGGGCAGATGGCAGTTAT
Indoleamine-dioxygenase 2	ido2	DLAgn_00014730^a	2.08	55	F: TGAAGGTGTGAGCAATGAGC R: CAAAGCACTGAATGGCTGAA

^aSequences obtained from databases dicLab v1.0c seabass genome.

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Table 3 Oligonucleotide sequences used to evaluate relative mRNA abundance of genes in the hypothalamus, pituitary gland and head-kidney by RT-qPCR, including forward (F) and reverse (R) primers, GenBank ID (NCBI), efficiencies (E) of qPCR reactions and annealing temperature (Ta).

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