Figure 4
From: Removal of dead fish eggs by Asellus aquaticus as a potential biological control in aquaculture
Effects of A. aquaticus presence during embryonic development on the locomotor activity of zebrafish larvae (E3). The figure summarizes the behavioral response of 120 hpf larvae developed in the presence or absence of A. aquaticus and/or dead zebrafish eggs during light–dark-light exposure. Under control conditions, the transition from light to dark elicits a sudden increase in the locomotor activity of zebrafish larvae. This activity decreases throughout the dark and the second light period. The whole test phase was divided into different periods to assess the potential alterations in the locomotor activity between the experimental groups. Locomotor activities were expressed as the distance traveled by the larvae with small or large movements. Line graphs showing the total distance covered by the larvae in small (A) and large (F) movements during a 10 min light—20 min dark—10 min light incubation with one minute resolution. Space between dashed lines (denoted as ‘1’, ‘2’, ‘3’, ‘4’) represents the statistically analyzed periods (300–600 s. (B,G) 600–660 s. (C,H) 660–1200 s. (D,I) 1200–1800 (E,J)) of the test. Data represent the mean and SD. Different letters indicate statistically significant differences, p < 0.05.
