Figure 1
Formation of a low cell density border between clonal colony fronts during swimming motility. Bacteria from the same colony were inoculated at 5 points in media containing 0.3% agar and incubated for 24 h at 37 °C, except for S. meliloti, which was incubated for 48 h at 30°C. All strains were inoculated in low nutrient media consisting of 10% LB (0.1% tryptone and 0.05% yeast extract), except for S. meliloti, which was inoculated in the nutrient rich medium, LBMC. An inhibitory border between colony fronts is observed, with the strongest effect seen with the center inoculant. Cells were inoculated in a quincunx pattern with each point being 1 cm from the center inoculant.
