Figure 6

Diffusible inhibitory compound causes damage to the bacterial cell membrane. Escherichia coli BW25113 was spotted onto an LB 0.8% agar (a, control) or in the center of a 1-day preincubated square streak of BW25113 (b). Plates were then incubated for 90 min and stained with cell permeable SYTO9 (green, live cells) and cell impermeable propidium iodide (PI, red cells, damaged membrane). Percentage of PI positive cells was determined from three independent fields of view from each condition (c).