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Figure 1

From: M1 macrophages polarized by crude polysaccharides isolated from Auricularia polytricha exhibit anti-tumor effect on human breast cancer cells

Figure 1

AP extraction, macrophage differentiation and cytotoxicity screening. (a) Mushroom extraction and crude polysaccharide isolation. (b) The polarization step (c) The morphological alteration of THP-1 derived macrophages after PMA activation captured by the phase-contrast microscope (scale bar; 100 μm). (d) Results from flow cytometry analysis showing the CD11b + surface marker expression after 48 h of PMA treatment (50 nM). (e) The cytotoxicity screening of THP-1-derived macrophages (M0) after 24 h of AP treatments; APH, APE, APW, and APW-CP. Data were expressed as mean ± SEM from triplicate experiments. The significant results were analyzed by Dunnett’s t-test, with * indicating P < 0.05 and ** indicating P < 0.001.

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