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Figure 1

From: Dual proteomics of infected macrophages reveal bacterial and host players involved in the Francisella intracellular life cycle and cell to cell dissemination by merocytophagy

Figure 1

Bacterial proteomics from proteomic approach. (A) Model of dual proteomic approach of infected cells by F. novicida. After 10 h of infection, infected cells with GFP-expressing bacteria were sorted and collected for proteomic analysis. (B) Bacterial proteins of Francisella in infected macrophages identified by proteomic method (C) Scatter plot of mean LFQ intensity of FRATN proteins found in infected macrophage (x axis) and in FRATN proteome (y axis), respectively after width adjustment normalization. In green and in red are displayed the proteins significantly down- and up-regulated, respectively, when Francisella infects macrophage. Significance was assessed with Student’s t-test on Perseus v1.6.15 with s0 = 1 and permutation-based FDR 1% used as a truncation method. (D) Scheme of biosynthesis of biotin and operon of biotin genes.

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