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Figure 2

From: Dual proteomics of infected macrophages reveal bacterial and host players involved in the Francisella intracellular life cycle and cell to cell dissemination by merocytophagy

Figure 2

Proteomic of infected cells by F. novicida and role of IRG1 in entry and intracellular multiplication of F. novicida. (A) Volcano plot representing the statistical comparison of the protein LFQ intensities of infected J774A.1 (right) vs non infected cells. Volcano was established using S0 = 0.1, FDR = 0.01. The abscissa reports the fold change in logarithmic scale (difference), the ordinate the − log(pvalue). (B) Volcano Plots representing the statistical comparison of the proteome of infected BMMs vs non infected cells (S0 = 0.1, FDR = 0.01). the abscissa reports the fold change in logarithmic scale (difference), the ordinate the − log(pvalue). Percentage of (C) entry of F. novicida and (D) kinetic of intracellular multiplication was monitored in J774A.1 macrophages transfected with siRNA of IRG1 over a 24 h-period in DMEM supplemented with glucose and 10% of FBS, and compared to that in J774A.1 WT macrophages. The expression of IRG1 was analyzed at each point of kinetics by qRT PCR. P < 0.01 (as determined by ANOVA test).

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