Figure 2
Effects of mare obesity and diet supplements on granulosa cell expression of mitochondrial proteins. Expression of mitochondrial complexes and antioxidant proteins in granulosa cells obtained from preovulatory follicles of normal weight (NW, n = 5), obese (OB, n = 5) and obese diet supplemented (OBD, n = 5) mares after ≥ 6 weeks of supplementation; data are presented as fold changes relative to the control group (NW): (a) representative western blot of electron transport system complexes I–V, (b) expression of subunits from each of the electron transport system complexes, (c) ROS producing complexes (I and III) relative to other complexes, (d) complex I relative to complex II, (e) complex V (ATP synthase) relative to other complexes (II, IV and V), and (f) the sum of all five complexes; (g) representative western blot of superoxide dismutase 2 (SOD2), superoxide dismutase 1 (SOD1), and glutathione peroxidase 1 (GPX1), (h) protein expression of SOD1, (i) SOD2, and (j) GPX1. Graphs represent mean ± SEM. Different superscripts indicate differences between groups at P < 0.05 (ab) and P < 0.1 (cd) using one-way ANOVA with post-hoc Tukey’s multiple comparison tests.
