Figure 4
Characterization of antagonist effects on the DP2 receptor sensor. Effect of increasing concentrations of DP2 receptor antagonist (A) fevipripant (B) AZD1981 and (C) ramatroban on the PGD2-activated DP2 receptor sensor activity. Concentration response relationships of PGD2 at DP2 receptor were investigated in the presence of either 30 nM, 100 nM or 1 µM of the respective tested antagonist. We used the PGD2 concentration response curve from Fig. 1 as a reference (sigmoidal fit shown as dashed line). The middle panel shows pEC50 for the individual experiments. The pEC50-values were used to calculate concentration-ratios (CR) for PGD2. The right panel shows the double log plot of (CR-1)-values dependent on the concentration of the indicated antagonist. The solid line shows the fitted linear regression. The slope values were 0.9993 (95% CI 0.312–1.687) for Ramatroban, 0.9991 (95% CI 0.544–1.454) for Fevipripant and 1.039 (95% CI 0.718–1.360) for AZD1981. The dashed line shows the respective linear regression with a slope constrained to 1 (for illustration purposes only). Data are presented as mean ± SEM.
