Figure 4
From: Modified polymeric biomaterials with antimicrobial and immunomodulating properties
Effect of the presence of CecA and Pur on the surface of PPmesh on the cell inflammatory response: NO secretion (Griess method) (A), COX-2 concentration (ELISA) (B), Th2-type immunological response cytokines (ELISA): IL-4 (C), IL-10 (D), and IL-13 (E), and inflammatory mediators panel (cytokine array) (F): MIF (F1), PAI-1 (F2), IL-6 (F3), and IL-8 (F4) levels; statistically significant results are indicated with ‘*’: *—p < 0.05, **—p < 0.01, ***—p < 0.001 (One-Way ANOVA, Tukey’s test). Control—PPmesh (unmodified), commercial prosthesis; CecA-PPmesh—prosthesis modified with cecropin A; Pur-PPmesh—prosthesis modified with puromycin; 1d/4d—cells cultured on the prostheses for 1 or 4 days; LPS(+) indicates cultures pre-incubated with 10 µg/ml LPS for 2 h.
