Table 1 Detection and performance characteristics for detection and quantification of non-protein amino acids: β-N-methylamino-L-alanine (BMAA), N-(2-aminoethyl)glycine (AEG), 2,4-diaminobutyric acid (DAB) and β-aminomethyl-L-alanine (BAMA) in American lobster (Homarus americanus). %RSD refers to relative standard deviation and MLOD is method limit of detection of the instrument.
Analyte | Retention Time (RT; min) | %RSD (RT) | Transition (m/z) | Cone Voltage (V) | Collision Voltage (V) | %RSD (signal) | MLOD (IUPAC) | % Recovery |
|---|---|---|---|---|---|---|---|---|
BAMA | 5.12 | 1.3 | 459 > 171 459 > 119 459 > 289 | 16 16 16 | 30 18 15 | 13.7 | 0.01 | 46.3 |
AEG | 5.56 | 1.1 | 459 > 171 459 > 119 459 > 289 459 > 214 | 16 16 16 16 | 30 18 15 20 | 10.9 | 0.01 | 41.3 |
BMAA | 5.78 | 1.2 | 459 > 171 459 > 119 459 > 289 459 > 258 | 16 16 16 16 | 30 18 15 20 | 18.7 | 0.005 | 68.8 |
DAB | 6.07 | 1.2 | 459 > 171 459 > 119 459 > 289 459 > 188 | 16 16 16 16 | 30 18 15 20 | 20.6 | 0.01 | 53.0 |
