Figure 3 | Scientific Reports

Figure 3

From: Genetic interference with HvNotch provides new insights into the role of the Notch-signalling pathway for developmental pattern formation in Hydra

Figure 3

Images and drawings of phenotypes observed in long-term sustained HvNICD-overexpressing transgenic Hydra. The images labeled with a–c represent light-microscopy, while a’–c’ display Ds-Red fluorescence. (A) “Ectopic tentacle” phenotype (a, b Ectoderm-TG; with ectopic tentacles indicated by white triangles). (B) “Multiple heads” with less ectopic heads compared to earlier stages (a Ectoderm-TG; b Endoderm-TG; the ectopic heads are numbered). (C) “two-headed” polyps with extra heads indicated by white arrows located in the lower part of the body column (a, b Ectoderm-TG; c endoderm-TG). (D) “Y-shaped” animals characterized by either shared feet or a shared head, which are indicated by blue arrows (a, c Ectoderm TG; b Endoderm-TG).

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