Figure 1
From: Regulation of translation in response to iron deficiency in human cells
Limitation of iron bioavailability in U-2 OS cell cultures arrests cell growth and promotes autophagy. U-2 OS cells were cultured for 3 days in the presence of the indicated concentrations of NaAsO2 or DFO. (A) Cell number was determined at the indicated time points. At least 3 independent biological replicates were performed, and the mean and standard deviation were calculated and represented. Different letters above bars indicate statistically significant differences (p-value < 0.02). (B) Total proteins were extracted, and P62, LC3B and GAPDH protein levels were determined by immunoblotting with specific antibodies. P62 and LC3B protein levels were expressed relative to those of GAPDH in order to normalize the results. The ratio LC3B-I/ LC3B-II is also indicated. The molecular weight (kDa) is represented on the right. A representative experiment of two independent biological replicates is shown.
