Figure 5
From: Regulation of translation in response to iron deficiency in human cells
4EBP1 is dephosphorylated in response to iron deficiency. U-2 OS cells were cultured and proteins were extracted as described in Fig. 4. Specific antibodies for total 4EBP1 and phosphorylated 4EBP1 (P-4EBP1) were used. The P-4EBP1/4EBP1 ratio is represented. The molecular weight (kDa) is indicated on the right. A representative experiment of two independent biological replicates is shown.
