Figure 6
From: Regulation of translation in response to iron deficiency in human cells
Iron deficiency does not promote stress granule assembly. (A) U-2 OS cells were cultured in the presence of NaAsO2 (1 h, 200 μM) or DFO (500 μM or 1 mM). DFO samples were analyzed at 12 h, 24 h 48 h and 72 h with similar results. Images from 2 days at 500 μM DFO are shown. (B) SG components are cytosolic under iron-depleted conditions. Cells were cultured in 500 μM DFO for 2 days and the subcellular localization of G3BP1, PABP and MEK1/2 was determined by immunofluorescence.
