Table 1 Primer sequences of genes.

Gene	Primers	Sequence	Amplicon size (bp)	Primer Tm (°C)
16S rRNA	Forward	5′–GAGTTTGATCCTGGCTCAG–3′	1490	55
16S rRNA	Reverse	3′–GGCTACCTTGTTACGA–5′	1490	55
cadA	Forward	5′–ATCACCATACCGGAAGCTGC–3′	623	57
cadA	Reverse	3′–TAGTGACCAGCAGCAGGCAG–5′	623	57
tcpA	Forward	5′–GAAGAAGTTTGTAAAAGAAGAACAC–3′	472	55.5
tcpA	Reverse	3′–GAAAGGACCTTCTTTCACGTTG–5′^17,18	472	55.5
UreC	Forward	5′–GTTATTCGTGATGGTATGGG–3′	317	57
UreC	Reverse	3′–GTAAAGGTGGTTACGCCAGA–5′¹⁹	317	57

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