Table 4 Selected gRNA of UreC and tcpA genes for CRISPR based editing.
gRNA | Target sequences | MIT Score | Off-target counts |
|---|---|---|---|
UreC | |||
27forward | GTTTTGTGCTGAGTGTGTCGATGT | 100 | 1 |
109forward | ATTAAAGATGGCCGTATTGTCGGT | 100 | 1 |
124forward | ATTGTCGGTATTGGCAAGGCAGGT | 100 | 1 |
180forward | CATTGGCCCCGGAACAGAAGTTGT | 100 | 1 |
214forward | CATTGGCCCCGGAACAGAAGTTGT | 100 | 1 |
tcpA | |||
35forward | CGATCGTGTGGTCATTGGCCAGGT | 100 | 1 |
120forward | TGCATTCGGCTCGCCGCCGGGGGT | 100 | 1 |
185forward | CCCGTACTTTTGTACGGGGTTTGT | 100 | 1 |
229forward | GGGTCAGCTCGTGACAACTATAGT | 100 | 1 |
