Figure 2 | Scientific Reports

Figure 2

From: Disruption of the pro-oncogenic c-RAF–PDE8A complex represents a differentiated approach to treating KRAS–c-RAF dependent PDAC

Figure 2

Targeted c-RAF–PDE8A disruption. (A) Immunofluorescent co-staining of endogenous c-RAF (mouse α-c-RAF, green) and PDE8A (rabbit α–PDE8A, red) proteins in fixed PANC1 cells. Nuclei counterstained with DAPI (blue) and composite highlighting areas of c-RAF–PDE8A colocalisation (n > 40 cells, scale bar = 20 µm). (B)(i), (ii) Proximity ligation assay (PLA) highlighting formation of c-RAF–PDE8A complex (red) within cytoplasm and nuclei of fixed PANC1 cells (scale bar = 20 µm). Disruption of c-RAF–PDE8A complex formation by (4 h) DRx-170, but not DRx-150 or vehicle (1% DMSO)–(ii) shown by dot plot (N = 3, n ≥ 90 cells per sample set). (C) RTCA (xCELLigence) analysis demonstrating how c-RAF–PDE8A disruption influences PANC1 cancer cell growth (N = 3). MEAN ± SEM, ns, not significant; **P < 0.01, ***P < 0.001; ****P < 0.0001.

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