Table 1 Oligonucleotides used in this study.
Primer name | Info | Sequence 5′–3′ | Target gene | Notes |
|---|---|---|---|---|
pdel_36543_5F | construction of deletion cassette | GTAACGCCAGGGTTTTCCCAGTCACGACGTGTACCTGTACCTTACCACG | ste12 | This study |
pdel_36543_5R | construction of deletion cassette construction of deletion cassette | ATCCACTTAACGTTACTGAAATCTCCAACGTGTGTGTGTGAGAGAGACC | ste12 | This study |
pdel_36543_3F | construction of deletion cassette | CTCCTTCAATATCATCTTCTGTCTCCGACTCCAGTGGGATAATACCTGC | ste12 | This study |
pdel_36543_3R | construction of deletion cassette | GCGGATAACAATTTCACACAGGAAACAGCTCTCCTATTACCTGTCTACG | ste12 | This study |
RT_36543_F | internal primer | CCACATCAGCGACGACAT | ste12 | This study |
RT_36543_R | internal primer | GAGTGAGACTTGTGAGGGTAAG | ste12 | This study |
EF1-728F | internal primer | CATCGAGAAGTTCGAGAAGG | tef1 | |
TEF1 rev | internal primer | GCCATCCTTGGAGATACCAGC | tef1 | |
RTcbh1F | qPCR primer | ACCGTTGTCACCCAGTTCG | cbh1 | |
RTcbh1R | qPCR primer | ATCGTTGAGCTCGTTGCCAG | cbh1 | |
RT_VEL_R1 | qPCR primer | GCAGGAACACCAGTCAGGATG | vel1 | |
RT_VEL_F1 | qPCR primer | CGAGGAGGGCAAGGACATTAC | vel1 | |
SAR RTF1 | qPCR primer | TGGATCGTCAACTGGTTCTACGA | sar | |
SAR RTR1 | qPCR primer | GCATGTGTAGCAACGTGGTCTTT | sar | |
RT_82208_F | qPCR primer | ACTGAAGCAGTATCGGGCAACT | pks4 | |
RT_82208_R | qPCR primer | TCTTCGACGTAAAGAGCAGCCA | pks4 | |
xyr1RTF | qPCR primer | CTTCCTCCTCCTGCTCATCG | xyr1 | |
xyr1RTR | qPCR primer | TCGTGTGCCCTAACAATGGTC | xyr1 | |
RT_CRE1 F | qPCR primer | GCAGCACAATACGACTCCG | cre1 | This study |
RT_CRE1 R | qPCR primer | CGGCTAATGATGTCGGTAAG | cre1 | This study |
