Figure 3 | Scientific Reports

Figure 3

From: Spatiotemporal expression of thyroid hormone transporter MCT8 and THRA mRNA in human cerebral organoids recapitulating first trimester cortex development

Figure 3

MCT8 is expressed in early neuroepithelium. (A–F) In 2-week-old organoids, membrane MCT8 staining is prominent in SOX2+/Nestin+ neuronal progenitor cells (NPC). Note that MCT8 expression was present in both NPCs organized in rosettes (A–C) and NPCs not yet arranged in a structured fashion (D–F). (G–H) At the onset of neurogenesis in 3-week-old organoids, membrane MCT8 staining was observed in SOX2+ NPCs in the neuroepithelium and the first few DCX+ newborn neurons. Note the enhanced MCT8 staining signal at the apical border of the neuroepithelium facing the ventricular lumen. (J–N) Mitotic RGC at the apical pole of the VZ of 4.5-week-old organoids maintain MCT8 expression. Analysis of different sectioning planes throughout the VZ (see M,N) shows a homogenous MCT8 immunostaining intensity of RGC. Scale bars: 50 µm (J), 20 µm (A–I,K–N).

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